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Persistence of Bluetongue virus serotype 1 virulence in sheep blood refrigerated for 10 years

Authors :
Giantonella, Puggioni
Davide, Pintus
Giorgio, Meloni
Rosario, Scivoli
Angela Maria, Rocchigiani
Daniela, Manunta
Giovanni, Savini
Annalisa, Oggiano
Ciriaco, Ligios
Source :
Veterinaria italiana. 54(4)
Publication Year :
2017

Abstract

This paper reports that Bluetongue virus serotype 1 (BTV-1) infected blood collected during the 2006 Sardinia (Italy) epidemic from a ewe with clinical disease and stored at ~ 5°C for 10 years, caused Bluetongue (BT)-like clinical disease and death when inoculated into a susceptible Sarda breed ram. Anatomo-histopathological examination and Real-Time Reverse Transcriptase PCR (Real-Time RT-PCR) confirmed the presence of BTV-1 in several tissues proving that the BTV-1 2006 isolate has maintained its infectivity and virulence.

Details

ISSN :
18281427
Volume :
54
Issue :
4
Database :
OpenAIRE
Journal :
Veterinaria italiana
Accession number :
edsair.pmid..........c04048946d720363d05d3eecfcf13f3f