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High-throughput confocal airy beam oblique light-sheet tomography of brain-wide imaging at single-cell resolution
- Source :
- bioRxiv
- Publication Year :
- 2023
- Publisher :
- Cold Spring Harbor Laboratory, 2023.
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Abstract
- Brain research is an area of research characterized by its cutting-edge nature, with brain mapping constituting a crucial aspect of this field. As sequencing tools have played a crucial role in gene sequencing, brain mapping largely depends on automated, high-throughput and high-resolution imaging techniques. Over the years, the demand for high-throughput imaging has scaled exponentially with the rapid development of microscopic brain mapping. In this paper, we introduce the novel concept of confocal Airy beam into oblique light-sheet tomography named CAB-OLST. We demonstrate that this technique enables the high throughput of brain-wide imaging of long-distance axon projection for the entire mouse brain at a resolution of 0.26 μm × 0.26 μm × 1.06 μm in 58 hours. This technique represents an innovative contribution to the field of brain research by setting a new standard for high-throughput imaging techniques.
- Subjects :
- Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- bioRxiv
- Accession number :
- edsair.pmid..........be25ece8c1ed878e7fa471108de4cfde