[Screening of interacting proteins of SHIP2 in human gastric mucosal epithelium by yeast two-hybrid system]

Objective To screen a standard homogenous cDNA library of human gastric mucosal epithelium with yeast two-hybrid system and find the proteins that interact with Src-homology 2-containing inositol 5-phosphatase 2 (SHIP2). Methods Using the yeast two-hybrid system, P1 (SH2+5-Ptase) and P2 (PRD+SAM) segments of SHIP2 were used as bait proteins to screen the proteins that bind to SHIP2 from the homogenous cDNA library of human gastric mucosal epithelium. The selected interacting proteins of SHIP2 were verified by co-immunoprecipitation assay. Results A total of 39 positive clones were selected and sequenced for alignment analysis. It was verified that PHB interacted with SHIP2 by reductive hybridization and co-immunoprecipitation assays. Conclusion PHB, the interacting protein of SHIP2 was screened by yeast two-hybrid system from the homogenous cDNA library of human gastric mucosal epithelium.