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Isolation and characterization of Chlorella viruses from freshwater sources in Korea
- Source :
- Molecules and cells. 14(2)
- Publication Year :
- 2002
-
Abstract
- We isolated 23 Chlorella viruses from 9 Korean cities. The viruses were initially amplified in the Chlorella strain NC64A. Pure isolates were obtained by repeated plaque isolations. A SDS-PAGE analysis revealed similar but distinct protein patterns, both among the group of purified viruses and in comparison with the prototype Chlorella virus PBCV-1. Digestions of the 330- to 350-kb genomic DNAs with 10 restriction enzymes revealed different restriction fragment patterns among the isolates. One isolate, SS-1, was resistant to digestion with HindIII, PvuII, AluI, and HaeIII, indicating methylation at the AGCT or GC sequences. Some isolates reacted with antiserum against PBCV-1. The others that did not react to this PBCV-1 antibody reacted to the antibody that was raised against purified HS-2 virion. The tRNA-coding regions of 8 Chlorella viruses were cloned and sequenced. These viruses contained 14-16 tRNA genes within a 1.2- to 2-kb region, except for the SS-1 isolate, which had a 1039-bp spacer in a cluster of 11 tRNA genes. The SS-1 spacer contained an open-reading frame (ORF) of 294 amino acids. This ORF had a 51% amino acid sequence similarity to the PBCV-1 ORF A478L. A Southern blot analysis suggested that it was a novel gene that lacked a homologue in PBCV-1.
Details
- ISSN :
- 10168478
- Volume :
- 14
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecules and cells
- Accession number :
- edsair.pmid..........bbd2adc39bc15928d4c44a9fb54b8d65