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- Source :
- Frontiers in Cellular and Infection Microbiology
- Publication Year :
- 2016
-
Abstract
- Escherichia coli strains belonging to serogroups O1 and O2 are frequently associated with human infections, especially extra-intestinal infections such as bloodstream infections or urinary tract infections. These strains can be associated with a large array of flagellar antigens. Because of their frequency and clinical importance, a reliable detection of E. coli O1 and O2 strains and also the frequently associated K1 capsule is important for diagnosis and source attribution of E. coli infections in humans and animals. By sequencing the O-antigen clusters of various O1 and O2 strains we showed that the serogroups O1 and O2 are encoded by different sets of O-antigen encoding genes and identified potentially new O-groups. We developed qPCR-assays to detect the various O1 and O2 variants and the K1-encoding gene. These qPCR assays proved to be 100% sensitive and 100% specific and could be valuable tools for the investigations of zoonotic and food-borne infection of humans with O1 and O2 extra-intestinal (ExPEC) or Shiga toxin-producing E. coli (STEC) strains.
- Subjects :
- DNA, Bacterial
Lipopolysaccharides
Extraintestinal Pathogenic Escherichia coli
Shiga toxin-producing E. coli
Serogroup
Microbiology
Foodborne Diseases
Zoonoses
Escherichia coli
Animals
Humans
Serotyping
Escherichia coli Infections
Phylogeny
Original Research
ExPEC
Shiga-Toxigenic Escherichia coli
Escherichia coli Proteins
extraintestinal E. coli
E. coli
Glycosyltransferases
Membrane Transport Proteins
O Antigens
Sequence Analysis, DNA
O-AGC
STEC
Multigene Family
O-antigen gene cluster
Subjects
Details
- ISSN :
- 22352988
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Frontiers in cellular and infection microbiology
- Accession number :
- edsair.pmid..........bb35a8187cba992c82e72be8ac523342