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Galectin-1 is implicated in the protein kinase C epsilon/vimentin-controlled trafficking of integrin-beta1 in glioblastoma cells
- Source :
- Brain Pathology (Zurich, Switzerland)
- Publication Year :
- 2008
-
Abstract
- Cell motility and resistance to apoptosis characterize glioblastoma (GBM) growth and malignancy. In our current work we report that galectin-1, a homodimeric adhesion molecule and carbohydrate-binding protein with affinity for beta-galactosides, is linked with cell surface expression of integrin beta1 and the process of integrin trafficking. Using immunofluorescence, depletion of galectin-1 through both stable knockdown and transient-targeted small interfering RNA (siRNA) treatment induces an intracellular accumulation of integrin-beta1 coincident with a diminution of integrin-beta1 at points of cellular adhesion at the cell membrane. Galectin-1 depletion does not alter the gene expression level of integrin-beta1. Transient galectin-1 depletion effectuates as well the perinuclear accumulation of protein kinase C epsilon (PKCepsilon) and the intermediate filament vimentin, both of which have been shown to mediate integrin recycling in motile cells. Our results argue for the involvement of galectin-1 in the PKCepsilon/vimentin-controlled trafficking of integrin-beta1. The understanding of molecular mediators such as galectin-1 and the pathways through which they drive the cell invasion so descriptive of GBM is anticipated to reveal potential therapeutic targets that promote glioma malignancy.
- Subjects :
- Trafficking
Galectin 1
Brain Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Integrin beta1
Blotting, Western
Genomics
Protein Kinase C-epsilon
Endoplasmic Reticulum
Transfection
Neoplasm Proteins
Antisense Elements (Genetics)
Cell Line, Tumor
Galectin-1
Humans
Vimentin
Gene Silencing
RNA, Small Interfering
Glioblastoma
Integrin alpha Chains
Research Articles
Migration
Subjects
Details
- ISSN :
- 17503639
- Volume :
- 20
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Brain pathology (Zurich, Switzerland)
- Accession number :
- edsair.pmid..........ba520e9dd37ee3db0312eb51cccda24e