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Validation of a HeLa Mx2/Luc reporter cell line for the quantification of human type I interferons
- Source :
- Pharmacology. 84(3)
- Publication Year :
- 2009
-
Abstract
- Although antiviral assays have been the most widely available biological assays for interferons (IFNs), they are less sensitive and provide considerable interassay variation. In this study, we demonstrate a new reporter cell line, which is based on HeLa cells transfected with a plasmid containing a human Mx2 promoter driving a luciferase (Luc) cDNA. To characterize the specific gene expression profiles induced by interferon alpha, we analyzed the microarray results of interferon response gene expression induced by IFN-alpha2a or IFN-alpha2b treatment with HeLa cells. We found that the Mx2 gene increased the most by treatment with both IFN-alpha2a and IFN-alpha2b. Based on this result, we designed a reporter cell line, HeLa-Mx2, suitable for determination of IFN-alpha. HeLa cells were stably transfected with the luciferase gene under the control of Mx2 promoter. The expression of luciferase can be easily measured for luminescence using a 96-well luminometer and has been correlated with the concentration of added IFN and cell density. In the validation results, our reporter cell line had specificity for type I IFN, but the significant effects of a number of other cytokines such as tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-2, IL-5, IL-6 and GM-CSF, or type II interferon (IFN-gamma) were not observed. Moreover, the robustness of our cell line is demonstrated by the lack of an effect of the HeLa-Mx2 cell culture's age on the performance of the reporter gene assay. The reporter gene assay demonstrated reproducible dose-response curves for IFN-alpha2a in the range of 1-10,000 IU/ml. The 95% confidential limit and total coefficient of variation estimates ranged between 96 and 116 and 10.51% in the reducible range mentioned above, respectively. In conclusion, we established a stable IFN-responsible HeLa-Mx2 cell line, which has advantages with regard to simplicity, selectivity, and reliability over conventional cytopathic effect reduction assays used to quantify IFN-alpha activity.
- Subjects :
- DNA, Complementary
Dose-Response Relationship, Drug
Gene Expression Profiling
Interferon-alpha
Reproducibility of Results
Interferon alpha-2
Transfection
Recombinant Proteins
Cell Line
Genes, Reporter
Chlorocebus aethiops
Luminescent Measurements
Animals
Humans
Cattle
Luciferases
Vero Cells
HeLa Cells
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 14230313
- Volume :
- 84
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Pharmacology
- Accession number :
- edsair.pmid..........ba41a66f471607d7cf562154883fb6f2