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Early lineage restriction and regional segregation during mammalian heart development

Authors :
Lescroart, Fabienne
Chabab, Samira
Lin, Xionghui
Rulands, Steffen
Paulissen, Catherine
Rodolosse, Annie
Auer, Herbert
Achouri, Younes
Dubois, Christine
Bondue, Antoine
Simons, Benjamin D
Blanpain, Cédric
Publication Year :
2014

Abstract

Cardiac development arises from two sources of mesoderm progenitors, the first heart field (FHF) and the second (SHF). Mesp1 has been proposed to mark the most primitive multipotent cardiac progenitors common for both heart fields. Here, using clonal analysis of the earliest prospective cardiovascular progenitors in a temporally controlled manner during early gastrulation, we found that Mesp1 progenitors consist of two temporally distinct pools of progenitors restricted to either the FHF or the SHF. FHF progenitors were unipotent, whereas SHF progenitors were either unipotent or bipotent. Microarray and single-cell PCR with reverse transcription analysis of Mesp1 progenitors revealed the existence of molecularly distinct populations of Mesp1 progenitors, consistent with their lineage and regional contribution. Together, these results provide evidence that heart development arises from distinct populations of unipotent and bipotent cardiac progenitors that independently express Mesp1 at different time points during their specification, revealing that the regional segregation and lineage restriction of cardiac progenitors occur very early during gastrulation.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.pmid..........b6cef574687b10d7b2a747a59542fe64