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Molecular cloning and characterization of murine interleukin-11
- Source :
- Experimental hematology. 24(12)
- Publication Year :
- 1996
-
Abstract
- Human interleukin-11 (IL-11) has been shown to have pleiotropic action on hematopoietic, hepatic, stromal, epithelial, neural, and osteoclast cells. In the present work, the murine IL-11 cDNA has been isolated from a fetal thymic cell line, and its structure and function compared with human IL-11. The murine protein was demonstrated to have identical actions on the proliferation of a murine plasmacytoma cell line, murine primitive bone marrow progenitor cells, and megakaryocyte precursors. The murine IL-11 protein was synthesized as a soluble thioredoxin-IL-11 fusion in Escherichia coli and the expression of murine IL-11 was examined by pulse-chase radiolabeling in COS cells. The chromosomal location of the murine IL-11 gene was assigned to the proximal arm of chromosome 7.
- Subjects :
- DNA, Complementary
Base Sequence
Molecular Structure
Molecular Sequence Data
Thymus Gland
Interleukin-11
Transfection
Cell Line
Mice, Inbred C57BL
Mice
Fetus
COS Cells
Animals
Humans
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Sequence Analysis
Polymorphism, Single-Stranded Conformational
Subjects
Details
- ISSN :
- 0301472X
- Volume :
- 24
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Experimental hematology
- Accession number :
- edsair.pmid..........b23a057f0c82ab49b1788cb80cebb9ed