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Molecular cloning and characterization of murine interleukin-11

Authors :
J C, Morris
S, Neben
F, Bennett
H, Finnerty
A, Long
D R, Beier
S, Kovacic
J M, McCoy
E, DiBlasio-Smith
E R, La Vallie
A, Caruso
J, Calvetti
G, Morris
N, Weich
S R, Paul
P S, Crosier
K J, Turner
C R, Wood
Source :
Experimental hematology. 24(12)
Publication Year :
1996

Abstract

Human interleukin-11 (IL-11) has been shown to have pleiotropic action on hematopoietic, hepatic, stromal, epithelial, neural, and osteoclast cells. In the present work, the murine IL-11 cDNA has been isolated from a fetal thymic cell line, and its structure and function compared with human IL-11. The murine protein was demonstrated to have identical actions on the proliferation of a murine plasmacytoma cell line, murine primitive bone marrow progenitor cells, and megakaryocyte precursors. The murine IL-11 protein was synthesized as a soluble thioredoxin-IL-11 fusion in Escherichia coli and the expression of murine IL-11 was examined by pulse-chase radiolabeling in COS cells. The chromosomal location of the murine IL-11 gene was assigned to the proximal arm of chromosome 7.

Details

ISSN :
0301472X
Volume :
24
Issue :
12
Database :
OpenAIRE
Journal :
Experimental hematology
Accession number :
edsair.pmid..........b23a057f0c82ab49b1788cb80cebb9ed