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OsPUB15, an E3 ubiquitin ligase, functions to reduce cellular oxidative stress during seedling establishment
- Source :
- The Plant journal : for cell and molecular biology. 65(2)
- Publication Year :
- 2011
-
Abstract
- The plant U-box (PUB) protein functions as an E3 ligase to poly-ubiquitinate a target protein for its degradation or post-translational modification. Here, we report functional roles for OsPUB15, which encodes a cytosolic U-box protein in the class-II PUB family. Self-ubiquitination assays showed that bacterially expressed MBP-OsPUB15 protein has E3 ubiquitin ligase activity. A T-DNA insertional mutation in OsPUB15 caused severe growth retardation and a seedling-lethal phenotype. Mutant seeds did not produce primary roots, and their shoot development was significantly delayed. Transgenic plants expressing the OsPUB15 antisense transcript phenocopied these mutant characters. The abnormal phenotypes were partially rescued by two antioxidants, catechin and ascorbic acid. Germinating seeds in the dark also recovered the rootless defect. Levels of H2O2 and oxidized proteins were higher in the knock-out mutant compared with the wild type. OsPUB15 transcript levels were increased upon H2O2, salt and drought stresses; plants overexpressing the gene grew better than the wild type under high salinity. These results indicate that PUB15 is a regulator that reduces reactive oxygen species (ROS) stress and cell death.
- Subjects :
- Base Sequence
Cell Death
Ubiquitin-Protein Ligases
Genetic Complementation Test
Molecular Sequence Data
Oryza
Hydrogen Peroxide
Salt Tolerance
Plants, Genetically Modified
Adaptation, Physiological
Plant Roots
Mutagenesis, Insertional
Oxidative Stress
Phenotype
Gene Expression Regulation, Plant
Seedlings
Seeds
Escherichia coli
Plant Proteins
Subjects
Details
- ISSN :
- 1365313X
- Volume :
- 65
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biology
- Accession number :
- edsair.pmid..........ad4dd081144d10c20cd2cd9c6353bb42