Expression of CD63 in Lung Tissue of Guinea Pigs Dying of Anaphylactic Shock

Objective To study the protein expression of cluster of differentiation 63 （CD63） in lung tissues of guinea pigs that died of anaphylactic shock and discuss the diagnostic value of CD63 for death from anaphylactic shock. Methods Twenty guinea pigs were randomly divided into control group, anaphylactic shock immediate death group, cold storage group （4 ℃ for 48 h） and frozen group （-20 ℃ for 7 d）. The animal model of guinea pigs that died of anaphylactic shock was established with human mixed serum injection. The expression changes of CD63 protein and CD63 mRNA in lung tissues were detected by hematoxylin-eosin （HE） staining, immunohistochemical staining, Western blotting, enzyme-linked immunosorbent assay （ELISA） and real-time RT-PCR. Results HE staining results showed congestion, and edema of lung tissues, and eosinophil infiltration in the anaphylactic shock groups. Western blotting analysis results showed that the expression of CD63 protein in the lung tissues of guinea pigs that died of anaphylactic shock was significantly higher than that in the control group （过敏性休克死亡豚鼠肺组织中CD63的表达.目的 研究过敏性休克死亡豚鼠肺组织中分化抗原簇63（cluster of differentiation 63，CD63）的蛋白表达，探讨CD63对过敏性休克死亡的诊断价值。 方法 20只豚鼠随机分为对照组，过敏性休克死亡即刻组、冷藏组（4 ℃贮藏48 h）、冷冻组（-20 ℃贮藏7 d）。采用人混合血清注射建立过敏性休克死亡豚鼠动物模型。应用苏木精-伊红（hematoxylin-eosin，HE）染色、免疫组织化学染色、Western印迹法、酶联免疫吸附测定（enzyme-linked immunosorbent assay，ELISA）和real-time RT-PCR检测肺组织中CD63蛋白及CD63 mRNA的表达变化。 结果 HE染色结果显示，各过敏性休克组肺组织淤血、水肿，嗜酸性粒细胞浸润。Western印迹法检测结果显示，过敏性休克豚鼠肺组织中CD63蛋白表达高于对照组（法医病理学；过敏反应；休克；肺；分化抗原簇63；豚鼠.