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LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress
- Source :
- Molecules and cells. 17(1)
- Publication Year :
- 2004
-
Abstract
- The CD53 antigen is a member of the tetraspanin membrane protein family that is expressed in the lymphoid-myeloid lineage. Its biological role remains unknown. Using microarrays, we identified CD53 as one of the principal genes up-regulated by exposure of macrophages to LPS. Northern blot analysis confirmed the induction of CD53 in RAW264.7 macrophages treated with LPS or SNAP (a nitric oxide donor). Cells stably transfected with sense CD53 cDNA had increased levels of intracellular GSH and lower levels of peroxide, and were more resistant to H2O2 and to UVB irradiation. Cells harboring antisense CD53 had the opposite properties. We propose that the induction of CD53 is a major mechanism by which macrophages protect themselves against LPS-induced oxidative stress and UVB irradiation.
- Subjects :
- Antigens, Differentiation, T-Lymphocyte
Lipopolysaccharides
DNA, Complementary
Time Factors
Cell Survival
Ultraviolet Rays
Blotting, Western
Tetraspanin 25
Transfection
Polymerase Chain Reaction
Cell Line
Mice
Antigens, CD
Animals
Nitric Oxide Donors
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Penicillamine
Biological Transport
Hydrogen Peroxide
gamma-Glutamyltransferase
Blotting, Northern
Flow Cytometry
Glutathione
Up-Regulation
Oxidative Stress
RNA
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 10168478
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecules and cells
- Accession number :
- edsair.pmid..........a4b64c9e209d135b86c00b2f16282522