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Differential adrenergic regulation of the gene expression of the beta-adrenoceptor subtypes beta1, beta2 and beta3 in brown adipocytes
- Publication Year :
- 2000
-
Abstract
- In brown adipocytes, fundamental cellular processes (cell proliferation, differentiation and apoptosis) are regulated by adrenergic stimulation, notably through beta-adrenergic receptors. The presence of all three beta-receptor subtypes has been demonstrated in brown adipose tissue. Due to the significance of the action of these receptors and indications that the subtypes govern different processes, the adrenergic regulation of the expression of the beta(1)-(,) beta(2)- and beta(3)-adrenoceptor genes was examined in murine brown-fat primary cell cultures. Moderate levels of beta(1)-receptor mRNA, absence of beta(2)-receptor mRNA and high levels of beta(3)-receptor mRNA were observed in mature brown adipocytes (day 6 in culture). Noradrenaline (norepinephrine) addition led to diametrically opposite effects on beta(1)- (markedly enhanced expression) and beta(3)-gene expression (full cessation of expression, as previously shown). beta(2)-Gene expression was induced by noradrenaline, but only transiently (
- Subjects :
- Male
Transcription, Genetic
RNA Stability
Adrenergic beta-Antagonists
Mice
Norepinephrine
Adrenergic Agents
Adipose Tissue, Brown
Adrenergic beta-2 Receptor Antagonists
Receptors, Adrenergic, beta
Cyclic AMP
Animals
RNA, Messenger
Cycloheximide
Adrenergic beta-2 Receptor Agonists
Cells, Cultured
Protein Kinase C
Dose-Response Relationship, Drug
Adrenergic beta-Agonists
Adrenergic beta-1 Receptor Antagonists
Cold Temperature
Gene Expression Regulation
Adrenergic beta-1 Receptor Agonists
Protein Biosynthesis
Receptors, Adrenergic, beta-3
Receptors, Adrenergic, beta-2
Receptors, Adrenergic, beta-1
Research Article
Half-Life
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid..........a4a44402573597169b8a0e74dcf5b8e6