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Germline 657del5 mutation in the NBS1 gene in breast cancer patients
- Source :
- International journal of cancer. 106(3)
- Publication Year :
- 2003
-
Abstract
- In this report the proportion of consecutive and familial breast cancer cases harboring the 657del5 of exon 6 of the NBS1 gene was determined to assess whether it is associated with the increased risk of breast cancer development. The study consisted of 3 groups of patients: a series of consecutive 150 patients with histologically confirmed breast cancer, diagnosed under the age of 50 in the city of Szczecin; a series of 80 breast cancer patients with a family history of breast cancer in their first-degree relatives; and a series of 530 consecutive individuals without the diagnosis of breast cancer selected at random by family doctors from the city of Szczecin. Molecular examination included allele-specific PCR assay for the common Slavic NBS1 mutation (657del5), LOH analysis using denucleotide CA repeat microsatellite markers, haplotype analysis and sequencing. The NBS1 founder mutation was detected in 2 of 150 (1.3%) consecutive breast cancer cases diagnosed under the age of 50 years; in 3 of 80 familial breast cancer cases (3.7%); and in 3 of 530 individuals (0.6%) from the general population. Examination of tumor DNA from patients with the NBS1 mutation (groups A and B) revealed loss of heterozygosity (LOH) in all cases. Additional haplotype analysis revealed that allelic loss affects specifically wild-type alleles. The majority of probands with breast cancer and the NBS1 mutation had a positive family history of breast cancer in their first-degree relatives. It appears that the 657del5 mutation in exon 6 of the NBS1 gene is responsible for the occurrence of a small but significant proportion of familial breast cancer patients.
- Subjects :
- Adult
Ovarian Neoplasms
Carcinoma, Ductal, Breast
DNA Mutational Analysis
Loss of Heterozygosity
Nuclear Proteins
Breast Neoplasms
Cell Cycle Proteins
DNA, Neoplasm
Exons
Middle Aged
Polymerase Chain Reaction
Neoplasm Proteins
Haplotypes
Biomarkers, Tumor
Humans
Female
Genetic Predisposition to Disease
Gene Deletion
Germ-Line Mutation
Aged
DNA Primers
Microsatellite Repeats
Subjects
Details
- ISSN :
- 00207136
- Volume :
- 106
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International journal of cancer
- Accession number :
- edsair.pmid..........a44a6068f3965ea71586b7540b1b428c