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Subtype specific roles of mitogen activated protein kinases in L6E9 skeletal muscle cell differentiation
- Source :
- Molecular and cellular biochemistry. 238(1-2)
- Publication Year :
- 2002
-
Abstract
- Role of mitogen activated protein kinases (MAPK) in skeletal muscle differentiation is not fully understood. We investigated subtype-specific functions and their interactions, if any, in the regulation of myogenic differentiation in L6E9 skeletal muscle cells. We show inhibition of extracellular signal-regulated kinase-1 and -2 (ERK-1/-2) and activation of p38 MAP kinase during the differentiation of L6E9 rat skeletal muscle cells under low serum condition. Inhibition of ERK-1/-2 activity dramatically enhanced differentiation as was evident from cellular morphology, expression of muscle differentiation specific marker proteins, suggesting that ERK-1/-2 activation may be inhibitory to initiation and progression of differentiation. In contrast, inhibition of p38 MAP kinase completely prevented differentiation; meaning p38 activation is required from the initiation till terminal differentiation of L6E9 cells. Moreover, inhibition of ERK-1/-2 activities enhanced the activation of p38 MAP kinase that resulted in enhancement of differentiation; whereas inhibition of p38 MAP kinase activity enhanced the ERK-1/-2 activities culminating in abrogation of differentiation. We conclude that ERK-1/-2 andp38 MAPkinase cascades oppositelyregulate each other's function(s) thereby regulating L6E9 skeletal muscle differentiation.
- Subjects :
- Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Time Factors
Blotting, Western
Cell Differentiation
p38 Mitogen-Activated Protein Kinases
Cell Line
Rats
Animals
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors
Mitogen-Activated Protein Kinases
Muscle, Skeletal
Signal Transduction
Subjects
Details
- ISSN :
- 03008177
- Volume :
- 238
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular biochemistry
- Accession number :
- edsair.pmid..........9ff76c9c0b17e4667fbc3f5f86b7c02b