['Acupuncture network drug'--transformation and application strategies of exosomes based on characteristics of network regulation of acupuncture therapy]

Although the basic mechanism of acupuncture-moxibustion has been revealed from many aspects, there are still many shackles in the transformation of the related research achievements. The transformation of academic achievements of experimental acupunctology is an urgent issue to be solved at present. Network regulation is the basic action mode of acupuncture therapy. In the present paper, we proposed that the "acupuncture network drug" could carry a variety of effective active ingredients which may be the core component of network regulation of acupuncture therapy. The "exosomes", polyvesicle derived from the intracellular lysosomal microparticles invagination, contain complex RNAs and proteins and exist in the body fluids and function in secreting abundant activate substances to participate in intercellular communication, which is the research hotspot in the field of frontier life science in the world. They play an important role in the diagnosis and treatment of diseases, and drug development, etc.. Our studies using rats with adjuvant arthritis and mice with sepsis displayed that after intraperitoneal administration of serum exosomes derived from normal animals receiving acupuncture intervention, an acupuncture-like analgesic effect and an anti-inflammatory effect were achieved, respectively. It is thus possible that acupuncture network drugs could be developed from serum exosomes secreted by exosome autogenous living cells after acupuncture intervention by virtue of the characteristics of low immunogenicity and may have great advantages in drug development and modification. It is also expected to provide new ideas for the transformation of experimental research results and to in depth give explanations about the underlying mechanisms of acupuncture therapy.针灸起效的基础作用机制虽已从多个方面被证实，但如何实现针灸基础研究的科研成果转化是目前亟待解决的问题。网络调节是针刺的基本作用模式，外泌体是目前世界前沿生命科学领域的研究热点，在疾病诊断、治疗、药物开发等方面具有重要的作用与优势。外泌体作为细胞间通讯的一种新方式，可能是针刺信息传递的重要载体，通过人工干预回输外泌体可产生“类针刺样”作用。借助外泌体自体活细胞分泌、免疫原性反应极低、内含物质多的特点，以及在药物开发与改造中的巨大优势，可从“针刺后血清外泌体”中开发“针刺网络药”。.