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[Cobalt-corrin oligonucleotide derivatives as reagents for selective cleavage of nucleic acids]
- Source :
- Bioorganicheskaia khimiia. 21(6)
- Publication Year :
- 1995
-
Abstract
- Techniques for preparing catalytically active corrin complexes of cobalt with monofunctional e-carboxyl- and e-amino groups in a macroligand are suggested. Two methods of the covalent attachment of such Co-corrin complexes to the terminal 3'- and 5'-phosphate groups of oligodeoxyribonucleotides were studied: the introduction of the complex to the oligonucleotide after oligonucleotide synthesis in aqueous solution and in the course of automated solid-phase oligonucleotide synthesis. Introducing of the Co-corrin complex during solid-phase synthesis was more efficient. It was demonstrated that the oligonucleotide probe thus obtained was able to act as a nuclease in the presence of ascorbic acid, the location of the clearage sites being determined by the addressing oligonucleotide.
Details
- ISSN :
- 01323423
- Volume :
- 21
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Bioorganicheskaia khimiia
- Accession number :
- edsair.pmid..........9b017ffdd4e2c4db2a4711f758081ef3