[Study on variation of actinoside C in leaves of Actinidia kolomikta with different growth periods by RP-HPLC]

To determine actinoside C in the leaves of Actinidia kolomikta with different growth periods.The separation was performed at 25 degrees C on ZORBAX Extend C18 column (4.6 mm x 250 mm, 5 microm), using amixture of methanol and water (51:49) as a mobile phase. The flow rate was 1.2 mL x min(-1), and the wavelength for measurement was 267 nm.The results showed that the contents of actinoside C in the leaves of A. kolomikta were variety in different growth periods. Actinoside C could reach its highest content in the middle ten days of June, then the content would decrease in the middle ten days of July slightly, it could reach their lowest content in the middle ten days of August.The optimal collective date for A. kolomikta are in the middle ten days of June.