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Alternative, out-of-frame runt/MTG8 transcripts are encoded by the derivative (8) chromosome in the t(8;21) of acute myeloid leukemia M2
- Source :
- Blood. 84(7)
- Publication Year :
- 1994
-
Abstract
- In the t(8;21) of acute myeloid leukemia (AML) M2, breakpoints are clustered on both chromosomes. The chromosome 21 breakpoint cluster region (bcr) falls within the runt locus, in the intron immediately downstream of the exons encoding an evolutionary conserved domain (the runt box). Transcripts in which the runt box is fused in frame to a novel sequence derived from chromosome 8 (MTG8) have been previously identified and have been assumed to constitute a critical leukemogenic event. Here we show physical linkage of the chromosome 8 bcr to the MTG8 locus. Unexpectedly, not only does the bcr map upstream of the most 5' MTG8 exon found in runt/MTG8 fusion transcripts, but it also maps upstream of a further 5' exon. In addition, we demonstrate the presence of alternative transcripts, originating from the der(8) chromosome, in which runt is out of frame with MTG8. Thus, runt truncation per se, rather than its fusion to MTG8, may be the crucial leukemogenic event.
- Subjects :
- Base Sequence
Sequence Homology, Amino Acid
Chromosomes, Human, Pair 21
Molecular Sequence Data
Restriction Mapping
Gene Expression
Nuclear Proteins
Exons
Translocation, Genetic
DNA-Binding Proteins
Alternative Splicing
Leukemia, Myeloid, Acute
RUNX1 Translocation Partner 1 Protein
Proto-Oncogene Proteins
Drosophila Proteins
Humans
Amino Acid Sequence
RNA, Messenger
RNA, Neoplasm
Sequence Alignment
Chromosomes, Human, Pair 8
DNA Primers
Transcription Factors
Subjects
Details
- ISSN :
- 00064971
- Volume :
- 84
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.pmid..........90542a347c3b06cadae180508b6b84b6