Evaluating the Efficiency of REPLI-g® Single Cell Kit for Trace DNA Amplification

Objective To evaluate the efficiency of REPLI-g® Single Cell Kit for sample DNA amplification, and explore its application value in forensic trace DNA amplification. Methods Three DNA extraction kits were selected to extract DNA from peripheral blood of 10 unrelated individuals. The DNA yield and purity of the three DNA extraction kits were compared. According to the results of comparison, one DNA sample was selected to concentrate and dilute, then used as the initial sample of whole genome amplification （WGA）. REPLI-g® Single Cell Kit was used to amplify the initial sample at the whole genome level. The amplification yield and amplification times were calculated, and the distribution of DNA fragments was detected by agarose gel electrophoresis. GoldenREPLI-g® Single Cell Kit应用于微量DNA扩增的效率评估.目的 评估REPLI-g® Single Cell Kit应用于检材DNA扩增的效率，探讨该试剂盒在法医学微量DNA扩增方面的应用价值。 方法 选取3种微量DNA提取试剂盒，对来自10名无关个体的外周血进行DNA提取，并对所提DNA的产量和纯度进行比较。根据比较结果选取1例DNA样本进行浓缩稀释作为全基因组扩增起始样本，使用REPLI-g® Single Cell Kit对起始样本进行全基因组扩增，计算扩增产量及扩增倍数，并进行琼脂糖凝胶电泳检测片段分布情况。使用Golden法医遗传学；短串联重复；全基因组扩增；多重置换扩增；微量检材.