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CTX-M-15 extended-spectrum beta-lactamases in Enterobacteriaceae in the intensive care unit of Tlemcen Hospital, Algeria
- Source :
- Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit. 18(4)
- Publication Year :
- 2012
-
Abstract
- The aim of this study was to detect extended-spectrum beta-lactamases (ESBL) in Enterobacteriaceae isolates in the intensive care unit (ICU) of Tlemcen hospital in north-western Algeria. Antimicrobial susceptibility testing, molecular typing, characterization of ESBL-encoding genes and the genetic environment, conjugation experiments and plasmid analysis were carried out. In all, 28 Enterobacteriaceae isolates were isolated from specimens recovered from patients in the ICU and 2 from surfaces of the unit. Of these, 11 isolates (4 Escherichia coli, 5 Klebsiella pneumoniae and 2 Enterobacter cloacae) produced ESBL of the CT-X-M-15 type. Molecular typing of the isolates showed the clonal nature of 4 K. pneumoniae isolates. The bla(CTXM-15) gene was genetically linked to insertion sequence lSEcp1B and was transferable by conjugation from 3 isolates. Regular monitoring of resistance mechanisms, the establishment of a prevention strategy, and more rational and appropriate use of antibiotics are needed.
- Subjects :
- Cross Infection
Enterobacteriaceae Infections
Bacteremia
Microbial Sensitivity Tests
DNA Fingerprinting
Polymerase Chain Reaction
beta-Lactamases
Bacterial Typing Techniques
Electrophoresis, Gel, Pulsed-Field
Intensive Care Units
Anti-Infective Agents
Enterobacteriaceae
Algeria
Drug Resistance, Bacterial
Cluster Analysis
Humans
Subjects
Details
- ISSN :
- 10203397
- Volume :
- 18
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit
- Accession number :
- edsair.pmid..........88cdfabca3bd7be3941fcab50499326a