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Quantification of TEL-AML1 transcript for minimal residual disease assessment in childhood acute lymphoblastic leukaemia
Quantification of TEL-AML1 transcript for minimal residual disease assessment in childhood acute lymphoblastic leukaemia
- Source :
- British journal of haematology. 114(2)
- Publication Year :
- 2001
-
Abstract
- Strategies currently used for residual disease detection in acute lymphoblastic leukaemia (ALL) rely on polymerase chain reaction (PCR) detection of immunoglobulin and T-cell receptor rearrangements. The TEL-AML1 fusion transcript, which is associated with t(12;21) (p13;q22), is found in 25% of childhood B-cell precursor ALL, and represents an interesting alternative target. We compared two methods for quantitating TEL-AML1 fusion transcripts: competitive PCR and real-time PCR. These techniques showed similar sensitivity (5 x 10(-5)) and reproducibility. Giving highly correlated results, both techniques can be conveniently used for TEL-AML1 transcript quantification. The constancy of TEL-AML1 expression was evaluated by measuring TEL-AML1 transcripts at different steps of the cell cycle, and in 21 cases of ALL at diagnosis. No major variation in TEL-AML1 expression was observed during the cell cycle or in 20/21 of the ALL patients. Residual disease was then determined after completion of induction therapy in 20 patients with a TEL-AML1-positive ALL. Seven patients out of 20 (35%) were still positive, including two patients with high level of residual blasts (close to or beyond 10(-2)). When comparison was possible, results obtained using TEL-AML1 quantification were in accordance with those obtained using T-cell receptor rearrangements analysis.
- Subjects :
- Male
Neoplasm, Residual
Oncogene Proteins, Fusion
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression
Infant
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Gene Rearrangement, T-Lymphocyte
Prognosis
Child, Preschool
Core Binding Factor Alpha 2 Subunit
Humans
Female
Child
Follow-Up Studies
Subjects
Details
- ISSN :
- 00071048
- Volume :
- 114
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- British journal of haematology
- Accession number :
- edsair.pmid..........68dc55399e53ed3a8df253cc1c1ddf3f