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Successful Application of CuAAC Click Reaction in Constructing
- Source :
- ACS applied bio materials. 4(3)
- Publication Year :
- 2022
-
Abstract
- Immuno-positron emission tomography (immuno-PET) is a rapidly growing imaging technique in which antibodies are radiolabeled to monitor their in vivo behavior in real time. However, effecting the controlled conjugation of a chelate-bearing radioactive atom to a bulky antibody without affecting its immunoreactivity at a specific site is always challenging. The in vivo stability of the radiolabeled chelate is also a key issue for successful tumor imaging. To address these points, a facile ultra-stable radiolabeling platform is developed by using the propylene cross-bridged chelator (PCB-TE2A-alkyne), which can be instantly functionalized with various groups via the click reaction, thus enabling specific conjugation with antibodies as per choice. The PCB-TE2A-tetrazine derivative is selected to demonstrate the proposed strategy. The antibody trastuzumab is functionalized with the
Details
- ISSN :
- 25766422
- Volume :
- 4
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- ACS applied bio materials
- Accession number :
- edsair.pmid..........679cf7433daeb6fc8e757e6dee5ffb15