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Induction of drug resistance and protein kinase C genes in A2780 ovarian cancer cells after incubation with antineoplastic agents at sublethal concentrations
- Source :
- Anticancer research. 22(6C)
- Publication Year :
- 2003
-
Abstract
- We examined the inducibility of drug resistance (MDR1, MRP1, LRP) and protein kinase C (PKC) isozyme (alpha, epsilon, eta, theta, tau, zeta) corresponding genes in A2780 ovarian cancer cells after a 24-hour treatment with adriamycin (ADR), camptothecin (CAM), etoposide (ETO) or vincristine (VCR). Sublethal concentrations of drugs were used to exclude short-term effects caused by selection. Cell cycle analysis was performed to identify possible correlation between resistance factors, PKC isozymes and proliferation. We found a mostly combined induction of MDR1, LRP, PKC tau and PKC zeta by CAM, ETO and VCR. PKC alpha, epsilon, eta and theta gene expression altered variably. Cell cycle analysis showed that A2780 cells responded with a marked G2/M arrest after a 24-hour treatment with CAM, ETO and VCR but an association between the induction of PKC isozymes corresponding genes and proliferation was not seen. Our analysis points to a possible link between atypical PKC tau/PKC zeta and MDR1/LRP in cytostatic stress response of cancer cells.
- Subjects :
- Ovarian Neoplasms
Dose-Response Relationship, Drug
Cell Cycle
Antineoplastic Agents
Drug Resistance, Multiple
Gene Expression Regulation, Enzymologic
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
Isoenzymes
Doxorubicin
Tumor Cells, Cultured
Humans
Camptothecin
Female
ATP Binding Cassette Transporter, Subfamily B, Member 1
Genes, MDR
Multidrug Resistance-Associated Proteins
Protein Kinase C
Etoposide
Vault Ribonucleoprotein Particles
Subjects
Details
- ISSN :
- 02507005
- Volume :
- 22
- Issue :
- 6C
- Database :
- OpenAIRE
- Journal :
- Anticancer research
- Accession number :
- edsair.pmid..........67179436d6f53b9dc8f1517ccc71fa84