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CRISPR-delivery particles targeting nuclear receptor-interacting protein 1 (
- Source :
- The Journal of biological chemistry. 293(44)
- Publication Year :
- 2018
-
Abstract
- RNA-guided, engineered nucleases derived from the prokaryotic adaptive immune system CRISPR-Cas represent a powerful platform for gene deletion and editing. When used as a therapeutic approach, direct delivery of Cas9 protein and single-guide RNA (sgRNA) could circumvent the safety issues associated with plasmid delivery and therefore represents an attractive tool for precision genome engineering. Gene deletion or editing in adipose tissue to enhance its energy expenditure, fatty acid oxidation, and secretion of bioactive factors through a “browning” process presents a potential therapeutic strategy to alleviate metabolic disease. Here, we developed “CRISPR-delivery particles,” denoted CriPs, composed of nano-size complexes of Cas9 protein and sgRNA that are coated with an amphipathic peptide called Endo-Porter that mediates entry into cells. Efficient CRISPR-Cas9–mediated gene deletion of ectopically expressed GFP by CriPs was achieved in multiple cell types, including a macrophage cell line, primary macrophages, and primary pre-adipocytes. Significant GFP loss was also observed in peritoneal exudate cells with minimum systemic toxicity in GFP-expressing mice following intraperitoneal injection of CriPs containing Gfp-targeting sgRNA. Furthermore, disruption of a nuclear co-repressor of catabolism, the Nrip1 gene, in white adipocytes by CriPs enhanced adipocyte browning with a marked increase of uncoupling protein 1 (UCP1) expression. Of note, the CriP-mediated Nrip1 deletion did not produce detectable off-target effects. We conclude that CriPs offer an effective Cas9 and sgRNA delivery system for ablating targeted gene products in cultured cells and in vivo, providing a potential therapeutic strategy for metabolic disease.
- Subjects :
- Gene Editing
Adipose Tissue, White
Cell Line
Nuclear Receptor Interacting Protein 1
Mice, Inbred C57BL
Genes, Reporter
Gene Targeting
Adipocytes
Animals
Humans
Clustered Regularly Interspaced Short Palindromic Repeats
Editors' Picks
CRISPR-Cas Systems
Energy Metabolism
Uncoupling Protein 1
Plasmids
Subjects
Details
- ISSN :
- 1083351X
- Volume :
- 293
- Issue :
- 44
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.pmid..........66a6017b996fcf038e2ff0ee6e9c01ad