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Encapsulation of individual pancreatic islets by sol-gel SiO2: a novel procedure for perspective cellular grafts
- Source :
- Journal of biotechnology. 100(3)
- Publication Year :
- 2002
-
Abstract
- Pancreatic rat islets are encapsulated by a siliceous layer deposited on the surface of single islets upon reaction with gaseous siliceous precursors. The process preserves original islet dimensions and does not suppress viability or function. The encapsulated material is homogeneously distributed on the islet surface, and layer thickness can be controlled in the 0.1-2.0 microm interval. Dynamic perfusion experiments with glucose stimulation were carried out in both encapsulated and non-encapsulated islets. Results were treated according to a kinetic model presented here for the analysis of perfusion data; the model tested by literature data, was used to substantiate the diffusion features of the siliceous layer, which does not affect mass transfer of insulin but which modifies the texture of the islet surface tissue. The clinical potential of silica encapsulation was demonstrated by in vivo experiments using encapsulated islets transplanted into diabetic rats. Transplantation was carried out in both inbred and outbred rats and indicated prolonged restoration of normal glycaemia levels and protection from immunological attack.
- Subjects :
- Cell Survival
Islets of Langerhans Transplantation
Biocompatible Materials
Membranes, Artificial
Silicon Dioxide
Models, Biological
Microspheres
Diabetes Mellitus, Experimental
Rats
Rats, Sprague-Dawley
Islets of Langerhans
Glucose
Rats, Inbred Lew
Culture Techniques
Insulin Secretion
Animals
Insulin
Computer Simulation
Gels
Subjects
Details
- ISSN :
- 01681656
- Volume :
- 100
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of biotechnology
- Accession number :
- edsair.pmid..........659f5a75ba2f13424e95182e84a99ff9