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[The detection of Toxoplasma gondii in abortion tissues of sheep using the polymerase chain reaction]
- Source :
- DTW. Deutsche tierarztliche Wochenschrift. 102(2)
- Publication Year :
- 1995
-
Abstract
- A polymerase chain reaction was applied to detect Toxoplasma gondii DNA in placental and fetal tissue samples of 47 unselected ovine abortions of the lambing season 1990/91 (Baden-Württemberg, Rhineland, Hesse). For the amplification a 190 bp or 223 bp sequence of the B1-gene of T. gondii was selected as the target sequence. Both sequences were detected in five abortions. All positive results were immunohistochemically confirmed using the peroxidase antiperoxidase technique (PAP-staining). Thus, in Germany, too, T. gondii infection in sheep during pregnancy should be considered as a possible cause of abortions, particularly in case of abortions of unknown genesis.
Details
- Language :
- German
- ISSN :
- 03416593
- Volume :
- 102
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- DTW. Deutsche tierarztliche Wochenschrift
- Accession number :
- edsair.pmid..........640552f238032d4f7879557998a4cc54