Back to Search
Start Over
Helper component-proteinase enhances the activity of 1-deoxy-D-xylulose-5-phosphate synthase and promotes the biosynthesis of plastidic isoprenoids in Potato virus Y-infected tobacco
- Source :
- Plant, cellenvironment. 38(10)
- Publication Year :
- 2014
-
Abstract
- Virus-infected plants show strong morphological and physiological alterations. Many physiological processes in chloroplast are affected, including the plastidic isoprenoid biosynthetic pathway [the 2C-methyl-D-erythritol-4-phosphate (MEP) pathway]; indeed, isoprenoid contents have been demonstrated to be altered in virus-infected plants. In this study, we found that the levels of photosynthetic pigments and abscisic acid (ABA) were altered in Potato virus Y (PVY)-infected tobacco. Using yeast two-hybrid assays, we demonstrated an interaction between virus protein PVY helper component-proteinase (HC-Pro) and tobacco chloroplast protein 1-deoxy-D-xylulose-5-phosphate synthase (NtDXS). This interaction was confirmed using bimolecular fluorescence complementation (BiFC) assays and pull-down assays. The Transket_pyr domain (residues 394-561) of NtDXS was required for interaction with HC-Pro, while the N-terminal region of HC-Pro (residues 1-97) was necessary for interaction with NtDXS. Using in vitro enzyme activity assays, PVY HC-Pro was found to promote the synthase activity of NtDXS. We observed increases in photosynthetic pigment contents and ABA levels in transgenic plants with HC-Pro accumulating in the chloroplasts. During virus infection, the enhancement of plastidic isoprenoid biosynthesis was attributed to the enhancement of DXS activity by HC-Pro. Our study reveals a new role of HC-Pro in the host plant metabolic system and will contribute to the study of host-virus relationships.
- Subjects :
- Chloroplasts
Base Sequence
Terpenes
Molecular Sequence Data
Potyvirus
Sequence Analysis, DNA
Plants, Genetically Modified
Plant Leaves
Chloroplast Proteins
Cysteine Endopeptidases
Viral Proteins
Plant Growth Regulators
Transferases
Two-Hybrid System Techniques
Tobacco
Plastids
Photosynthesis
Abscisic Acid
Gene Library
Plant Diseases
Sequence Deletion
Subjects
Details
- ISSN :
- 13653040
- Volume :
- 38
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Plant, cellenvironment
- Accession number :
- edsair.pmid..........628fb5a4f0570cc57fb81c08649aa9b1