[Establishment and biological characteristics of two murine hepatocarcinoma substrains with different lymphatic metastatic ability]

The aim of the experiment is to establish two murine hepatocarcinoma (HepA cell line) substrains with different lymphatic metastatic ability and compare their biological characteristics. Two murine hepatocarcinoma substrains, HepA-H and HepA-L, were established by the cell cloning and the selection way of lymph node metastasis. After tumor cells were injected subcutaneously to foot pad of NIH mice, the popliteal lymph node metastatic rates of HepA-H and HepA-L were 83.3% and 16.7% respectively, which has statistical significance (p0.01). There were no metastatic lesions were found in other organs, such as lung, liver, spleen and kidney. In vitro, HepA-H showed a higher ability in growth, migration and adhesion than that of HepA-L. The establishment of two murine hepatocarcinoma substrains provides a useful animal model for the study of mechanism of the lymphatic metastasis.