The VRAC blocker DCPIB directly gates the BK channels and increases intracellular Ca

The volume regulated anion channel (VRAC) is known to be involved in different aspects of cancer cell behaviour and response to therapies. For this reason, we investigated the effect of DCPIB, a presumably specific blocker of VRAC, in two types of cancer: pancreatic duct adenocarcinoma (PDAC) and melanoma.We used patch-clamp electrophysiology, supported by CaDCPIB markedly increased whole-cell currents in Panc-1, MiaPaca2 and IGR39, but not in IGR37 cells. The currents were mostly mediated by KDCPIB directly targeted BK channels and, also, acutely increased intracellular Ca