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[Expression Profile of lncRNA NONHSAT040475 in Peripheral Blood Leukocytes of Healthy Persons]
- Source :
- Zhongguo shi yan xue ye xue za zhi. 24(4)
- Publication Year :
- 2016
-
Abstract
- To investigate the expression profile of lncRNA NONHSAT040475 in peripheral blood leukocytes of healthy persons.The peripheral blood mononuclear cells were collected from 10 healthy volunteers, the CD3(+) T cells,CD19(+) B cells,CD56(+) NK cells and granulocytes were purified and sorted by flow cytometry. Then, the expression of lncRNA NONHSAT040475 in peripheral blood leukocyte subsets was detected by real-time quantitative PCR.the expression levels of lncRNA NONHSAT040475 were various in lymphocyte subsets, its expression in B lymphocytes was significantly higher, as compared with T lymphocytes, while the expressions of lncRNA NONHSAT040475 in NK cells and granulocytes were relative low(P0.01).lncRNA NONHSAT040475 is widely expressed in human peripheral blood leukocytes, and mainly expressed in B lymphocytes, therefore, laying the foundation for further study of B lymphocyte-related diseases. This study has brought a new opportunity for diagnosing and illustrating the molecular mechanism of hematologic disorder or autoimmune disease.
Details
- ISSN :
- 10092137
- Volume :
- 24
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Zhongguo shi yan xue ye xue za zhi
- Accession number :
- edsair.pmid..........60776313edb7185c298f9bfaf201de21