Back to Search Start Over

[Expression of CSN Complex in ATRA-induced APL Cell Differentiation and Its Clinical Significance]

Authors :
Shu-Yuan, Liu
La-Gen, Wan
Lin-Lin, Gao
Yun-Yuan, Kong
Xin, Li
Zhang-Lin, Zhang
Source :
Zhongguo shi yan xue ye xue za zhi. 23(5)
Publication Year :
2015

Abstract

To investigate the expression of CSN complex (COP9 signal some subunits) in the patients with acute promyelocytic leukemia (APL) and its significance in the ATRA-induced APL differentiation.Using the NB4 cells as a model, morphologic observation and myeloid differentiation marker CD11b detection were used to monitor ATRA-induced APL differentiation, the expression of CSN complex in cell differentiation was detected by Western blot and reverse transcription real time fluorescent quantitative PCR (RT-qPCR) method. RT-qPCR was also used to detect the relative expression level of COP9 signalosome subunits in the APL patients and remission after treatment.ATRA could obviously enhance CD11b expression; the cell morphology showed obvious differentiation characteristics. During the differentiation, the expression of COP9 signalosome subunits was down-regulated by ATRA. Meanwhile, the CSN expression level in newly diagnosed APL patients was much higher than that in controls (non-leukemia) (P0.05). The level of CSN expression was obviously down-regulated when APL patients achieved complete remission.The high CSN expression level in APL patients can be down-regulated by ATRA. CSN complex may have a significant effect on the pathogenesis and therapy of APL.

Details

ISSN :
10092137
Volume :
23
Issue :
5
Database :
OpenAIRE
Journal :
Zhongguo shi yan xue ye xue za zhi
Accession number :
edsair.pmid..........5ec387cad6049500a7558e0e229909a3