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Purification and some properties of peroxidase isozymes from pineapple stem
- Source :
- Biochemistry and molecular biology international. 29(1)
- Publication Year :
- 1993
-
Abstract
- The enzyme peroxidase is widely distributed among the higher plants. Isozymes of peroxidase are known to occur in a variety of tissues in a large number of plant species. In this study, peroxidase isozymes were purified from the extract of pineapple stem through successive steps of ammonium sulfate fractionation, CM-Sepharose CL-6B chromatographies and DEAE-Sepharose CL-6B chromatographies. By these steps, twelve isozymes of peroxidase were obtained. Some properties of the isozymes were studied and compared.
- Subjects :
- Isoenzymes
Kinetics
Peroxidases
Ammonium Sulfate
Fruit
Hydrogen-Ion Concentration
Subjects
Details
- ISSN :
- 10399712
- Volume :
- 29
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemistry and molecular biology international
- Accession number :
- edsair.pmid..........5ad2434cd1fa6e556085a80fa90bd8fc