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[Regulating effect of anodonta glucan HBP-A on chondrocytes through Wnt pathway]
- Source :
- Zhongguo gu shang = China journal of orthopaedics and traumatology. 27(6)
- Publication Year :
- 2014
-
Abstract
- To investigate regulation function of anodonta glucan HBP-A on chondrocytes through Wnt pathway in vitro.Rat chondrocytes were cultured and differentiated induced with IL-1beta (10 ng/ml) in vitro. Chondrocytes were divided into five groups:IL-13 group,IL-1beta + IWP-2 (5 microM,Wnt pathway inhibitor) group, IL-1beta + HBP-A (0.3 mg/ml) group and IL-1beta + IWP-2 + HBP-A group. Wnt-3a, beta-catenin (24 h,48 h,72 h) and MMP-13(72 h) genes expression were detected by Rt-PCR, while beta-catenin, MMP-13, Sox-9 and coll-II (48 h) protein expression were measured by Western-blot.After induction of IL-1beta, gene expression of Wnt-3a, beta-catenin and MMP-13 were increased,so were the protein expression of beta-catenin and MMP-13. In contrast,protein expression of Sox-9 and Coll-II were declined. Following addition of HBP-A, Wnt-3a, beta-catenin and MMP-13 were shown as induction of IL-1beta, but protein expression of Sox-9 and Coll-II were upgraded. Combining HBP-A with IWP-2 led to the lowest level in Wnt-3a, beta-catenin gene and beta-catenin protein expression and highest expression of Sox-9 protein.HBP-A could not only delay the differentiation of chondrocytes through downgrading the signal expression of Wnt/beta-catenin,but also adjust the expression of Wnt-3a, beta-catenin and Sox-9 when combinated with the Wnt inhibitor.
Details
- ISSN :
- 10030034
- Volume :
- 27
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Zhongguo gu shang = China journal of orthopaedics and traumatology
- Accession number :
- edsair.pmid..........5a5e7bce50ba8ba71a207ca8633bb99c