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Characterization of Calcium-Binding Proteins from Parasitic Worms

Authors :
Charlotte M, Thomas
David J, Timson
Source :
Methods in molecular biology (Clifton, N.J.). 1929
Publication Year :
2019

Abstract

Parasitic diseases caused by helminths (worms) represent a major burden on humanity with hundreds of millions of people infected worldwide. However, there are relatively few drugs to treat these diseases, and resistance is emerging to some of these. Therefore, there is a pressing need to characterize proteins from helminths as potential drug targets. Calcium signalling proteins represent attractive targets due to the vital nature of properly regulated calcium-mediated signalling and the presence of unusual calcium-binding proteins in helminths. Here we present methods to characterize these proteins in terms of their ion-binding properties, drug-binding properties, and oligomeric state, including a method to correct for the effects of non-spherical proteins in analytical gel filtration. In addition we present an overview of their recombinant expression and purification and methods to predict their structures.

Details

ISSN :
19406029
Volume :
1929
Database :
OpenAIRE
Journal :
Methods in molecular biology (Clifton, N.J.)
Accession number :
edsair.pmid..........588186878d522b58b3fe437a416324b5