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Adaptor gamma ear homology domain conserved in gamma-adaptin and GGA proteins that interact with gamma-synergin
- Source :
- Biochemical and biophysical research communications. 271(3)
- Publication Year :
- 2000
-
Abstract
- We identified a novel family of proteins that have a VHS domain and an AGEH (adaptor gamma ear homology) domain that is homologous to the ear domain of the gamma-adaptin subunit of the AP-1 clathrin adaptor. When overexpressed, the proteins, called GGA1, GGA2, and GGA3, localized to the trans-Golgi network (TGN) and often caused fragmentation and vacuolation of the compartment. Yeast two-hybrid analysis showed that the AGEH domains of the GGA proteins as well as those of gamma-adaptins are able to interact with gamma-synergin, which was previously shown to localized in the TGN region and interact with gamma-adaptin. Furthermore, gamma-synergin and either of the GGA proteins coexpressed were colocalized in the TGN region. These results suggest that the GGA proteins regulate the function of the TGN or membrane trafficking from this compartment and that the AGEH domains of GGAs and gamma-adaptins, like the ear domain of alpha-adaptin, are involved in interaction with molecules that modulate their functions.
- Subjects :
- ADP-Ribosylation Factors
Adaptor Protein Complex 1
Molecular Sequence Data
Fluorescent Antibody Technique
Golgi Apparatus
Membrane Proteins
Proteins
Biological Transport
Transfection
Adaptor Proteins, Vesicular Transport
Hemagglutinins
Yeasts
Humans
Amino Acid Sequence
Cloning, Molecular
Carrier Proteins
Peptides
Adaptor Protein Complex gamma Subunits
Oligopeptides
Sequence Alignment
Conserved Sequence
HeLa Cells
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 271
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.pmid..........585a0ffe7fdf310f5efb574284b0d8db