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Characterization of paired Ig-like receptors in rats

Authors :
G, Dennis
R P, Stephan
H, Kubagawa
M D, Cooper
Source :
Journal of immunology (Baltimore, Md. : 1950). 163(12)
Publication Year :
1999

Abstract

To explore the phylogenetic history of the murine paired Ig-like receptors of activating (PIR-A) and inhibitory (PIR-B) types, we isolated PIR homologues from a rat splenocyte cDNA library. The rat (ra) PIR-A and raPIR-B cDNA sequences predict transmembrane proteins with six highly conserved extracellular Ig-like domains and distinctive membrane proximal, transmembrane, and cytoplasmic regions. The raPIR-B cytoplasmic region contains prototypic inhibitory motifs, whereas raPIR-A features a charged transmembrane region and a short cytoplasmic tail. Southern blot analysis predicts the presence of multiple Pira genes and a single Pirb gene in the rat genome. Although raPIR-A and raPIR-B are coordinately expressed by myeloid cells, analysis of mRNA detected unpaired expression of raPIR-A by B cells and raPIR-B by NK cells. Collectively, these findings indicate that the structural hallmarks of the Pir gene family are conserved in rats and mice, yet suggest divergence of PIR regulatory elements during rodent speciation.

Details

ISSN :
00221767
Volume :
163
Issue :
12
Database :
OpenAIRE
Journal :
Journal of immunology (Baltimore, Md. : 1950)
Accession number :
edsair.pmid..........56c547e06e32ea3470d45c25e15ff184