[Effects of fluoride on ionized calcium and calcium channel in osteoblast-like cell in vitro]

To study the effects of fluoride (F-) on ionized calcium ([Ca2+]i) and calcium channel in osteoblast-like cell (OBLs).Investigating [Ca2+]i was by fluorospectrophotometry and recording the calcium currents of OBLs was using whole patch-clamp technique.The addition of fluoride to the medium made a rapid and significant increase in free [Ca2+]i, especially in 100 ng F- /ml group. There were significances between control group and 50, 100 ng F- /ml groups. And 25 ng/ml F- could stimulate OBLs calcium channel open. Compared with control group, fluoride could significantly increase the amplitudes of calcium currents (P0.01), furthermore the activation was in dose-dependent manner.Fluoride can make calcium channel open and then increase the concentrations of [Ca2+]i.