Back to Search
Start Over
Complementation analysis with new genetic markers in Phaffia rhodozyma
- Source :
- Antonie van Leeuwenhoek. 73(3)
- Publication Year :
- 1998
-
Abstract
- Isolation and characterization of auxotrophic mutants from wild-type and astaxanthin mutant strains of Phaffia rhodozyma is described. Differences in survival were observed when u.v. irradiation of P. rhodozyma wild-type and astaxanthin mutant strains were incubated in the dark or exposed to photoreactivating light. Ultra-violet mutagenesis was not effective to produce auxotrophic mutants in this yeast. Auxotrophic mutants were obtained with high efficiency through a nystatin enrichment procedure after a N-methyl-N'-nitro-N-nitrosoguanidine (NGT) mutagenic treatment with a 0.12% survivor level. Stringent mutagenetic conditions were needed to obtain P. rhodozyma auxotrophs. The most frequent mutants were ade- and met- in a rather narrow auxotroph spectrum. These results may be associated with a possible diploid condition of this yeast. The high number of adenine auxotrophs obtained in relation to other auxotrophic mutants suggests the possibility of some degree of heterozygosity in the wild-type strain UCD 67-385.
Details
- ISSN :
- 00036072
- Volume :
- 73
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Antonie van Leeuwenhoek
- Accession number :
- edsair.pmid..........5362e72188a51bc5b5d95b49833e4348