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Virus-Induced Gene Silencing in Sorghum Using Brome Mosaic Virus
- Source :
- Methods in molecular biology (Clifton, N.J.). 2408
- Publication Year :
- 2022
-
Abstract
- Sorghum [Sorghum bicolor (L.) Moench.] is a versatile crop, grown in 30 countries and a food source for nearly 500 million people globally. Although the sorghum genome is sequenced, a limited understanding of gene function prevents the improvement of resistance against almost 150 species of viruses, bacteria, fungus, and parasitic plants to improve productivity. Here, we present a Brome mosaic virus (BMV)-based virus-induced gene silencing (VIGS) to silence target genes for functional study in sorghum. This protocol achieves 100% sorghum infection with BMV by growing the plants at 18 °C instead of 22 °C. Using this method, one can achieve gene silencing in sorghum up to 100% of the inoculated plants.
- Subjects :
- Humans
Gene Silencing
Edible Grain
Bromovirus
Software
Sorghum
Subjects
Details
- ISSN :
- 19406029
- Volume :
- 2408
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Accession number :
- edsair.pmid..........527693bc6eddc99954ee078c6bb19286