Functional Analysis of Foxp3 and Its Mutants by Retroviral Transduction of Murine Primary CD4

Authors :: Akira, Nakajima
Ryuichi, Murakami
Shohei, Hori
Source :: Methods in molecular biology (Clifton, N.J.). 2559
Publication Year :: 2022
Abstract: The transcription factor Foxp3/FOXP3 orchestrates regulatory T (Treg) cell development and function by interacting with numerous target genes and partner proteins. Functional analysis of naturally occurring or engineered Foxp3/FOXP3 mutations has provided important insights into how the complex Foxp3/FOXP3-centered molecular network operates. Here, we describe detailed protocols for retroviral transduction of murine primary conventional CD4

Subjects :: CD4-Positive T-Lymphocytes
Mice
Retroviridae
Gene Expression Regulation
Animals
Forkhead Transcription Factors
T-Lymphocytes, Regulatory

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