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Up-regulation of E-cadherin by saRNA inhibits the migration and invasion of renal carcinoma cells
- Source :
- International journal of clinical and experimental pathology. 11(12)
- Publication Year :
- 2018
-
Abstract
- Previous studies have reported that double stranded RNAs (dsRNAs) have a potent ability to induce gene expression by targeting its promoter in cancer cells, which is called RNA activation (RNAa). In the present study, we have identified that a candidate dsRNA (dsEcad-215) could stimulate E-cadherin mRNA and protein expression via RNAa in renal cell carcinoma (RCC). Because the expression level of E-cadherin was down-regulated in RCC tissues compared to adjacent non-tumor tissues, dsEcad-215 was subsequently transfected into the RCC cell lines ACHN and 786-O. Expectedly, our results indicated that transfection of dsEcad-215 readily inhibited cell migration and invasion. In addition, several critical EMT-promoting genes (ZEB-1 and Vimentin) were down-regulated, while the anti-EMT gene β-catenin was up-regulated both at the mRNA and protein levels after dsEcad-215 transfection, suggesting that an enhanced E-cadherin level by dsEcad-215 suppressed EMT to inhibit cell motility. Collectively, our findings provide a potential effective therapeutic strategy for RCC, and dsEcad-215 might act as an alternative anti-cancer metastasis drug.
- Subjects :
- Original Article
Subjects
Details
- ISSN :
- 19362625
- Volume :
- 11
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- International journal of clinical and experimental pathology
- Accession number :
- edsair.pmid..........4cb25b44b7142ba9421804e9a4185251