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Identification of genes required for immortalization in human papillomavirus-infected human oral keratinocytes
- Source :
- Cellular and molecular biology (Noisy-le-Grand, France).
- Publication Year :
- 2003
-
Abstract
- The early stages of head and neck cancer are presumed to require a senes of genetic alterations that are not represented by a distinct clinical phenotype. Therefore, genes with altered expression in the preneoplasia may be useful for the early detection of this highly recurrent cancer. In this study, we immortalized normal human oral keratinocytes (NHOK) by retroviral-mediated infection of HPV 16 transforming oncogenes, E6 and E7 (HOK16E6E7). Using the Affymetrix gene chip (U95Av2), we identified 177 known genes and EST that were overexpressed at least 3-fold or above in the immortalized cells, while 133 were down-regulated compared to NHOK. Northern blot analysis showed elevated levels of p55CDC in the immortalized cells, while NHOK showed high basal expression of small proline rich protein (SPRR2). The altered expression of these genes maybe associated with cellular proliferation or differentiation and the early stages of oral carcinogenesis.
- Subjects :
- DNA Replication
Keratinocytes
Papillomavirus Infections
Mouth Mucosa
Cell Cycle Proteins
Oncogenes
Blotting, Northern
Enzymes
Gene Expression Regulation, Neoplastic
Tumor Virus Infections
Cell Transformation, Neoplastic
Head and Neck Neoplasms
Humans
Cloning, Molecular
Growth Substances
Cell Adhesion Molecules
Papillomaviridae
Cells, Cultured
Subjects
Details
- ISSN :
- 01455680
- Database :
- OpenAIRE
- Journal :
- Cellular and molecular biology (Noisy-le-Grand, France)
- Accession number :
- edsair.pmid..........489ee14a07cc31aba35d26870f406021