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Gene-carried chitosan-linked polyethylenimine induced high gene transfection efficiency on dendritic cells
- Source :
- Biotechnology and applied biochemistry. 59(5)
- Publication Year :
- 2012
-
Abstract
- A dendritic cell (DC) networking system has become an attractive approach in cancer immunotherapy. Successful DC gene engineering depends on the development of transgene vectors. A cationic polymer, chitosan-linked polyethylenimine (PEI) (CP), possessing the advantages of both PEI and chitosan, has been applied in nonviral transfection of DCs. Physicochemical evaluation showed that CP/DNA complexes could form cationic nanoparticles. Compared with DCs transfected with commercial reagent, Lipofectamine2000, it showed higher transfection efficiency and lower cytotoxicity when DCs were transfected with CP/DNA complexes. A nuclear trafficking observation of CP/DNA complexes by a confocal laser scanning microscope further revealed that the CP could help DNA enter into the cytoplasm and finally into the nucleus of a DC. Finally, vaccination of DCs transfected with CP/DNA encoding gp100 slightly improved resistance to the B16BL6 melanoma challenge. This is the first report that CP polymer is used as a nonviral vector for DC gene delivery and DC vaccine. Essentially, these results might be helpful to design a promising nonviral vector for DC gene delivery.
Details
- ISSN :
- 14708744
- Volume :
- 59
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Biotechnology and applied biochemistry
- Accession number :
- edsair.pmid..........469b9758e5006071c4d32a0549145ce2