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Collection and Cryopreservation of Plasmodium falciparum Clinical Isolates in the Field

Collection and Cryopreservation of Plasmodium falciparum Clinical Isolates in the Field

Authors :
Frederica Dedo, Partey
Augustina, Frimpong
Michael Fokuo, Ofori
Source :
Methods in molecular biology (Clifton, N.J.). 2470
Publication Year :
2022

Abstract

P. falciparum causes the most severe form of malaria in younger children and pregnant women. In vitro culture systems allow researchers to understand parasite biology, elucidate mechanism of host immunity and test efficacy of antimalarial agents or vaccines in preclinical studies. Most laboratory-adapted parasite strains predate the emergence of artemisinin-based drug combinations and mainly originate from Asia or Europe. To fully understand the biochemical and phenotypic characteristics of parasites, it is imperative that researchers are able to culture parasites circulating in an area to unravel any geographical differences at the population level. Ex vivo culturing of clinical isolates can be challenging when collecting samples in the field and requires technical expertise and equipment. To overcome this challenge, clinical isolates are cryopreserved in the field and transported to a laboratory for in vitro studies. In this protocol, we describe different methods of cryopreserving P. falciparum isolates in the field and thawing them for subsequent in vitro culture.

Details

ISSN :
19406029
Volume :
2470
Database :
OpenAIRE
Journal :
Methods in molecular biology (Clifton, N.J.)
Accession number :
edsair.pmid..........462f0050201ed8c4ad3ea1ca91bab39d