[Authenticate of Atractylodes macrocephala seed by amplification refractory mutation system]

To design specific primers and authenticate Atractylodes macrocephala from Atractylodes lancea and A. chinensis.SNPs in the psbA-trnH sequences of Atractylodes were found by ClustulW program and Bioedit software. Primers for authentic A. macrocephala is designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system.172 bp band special for A. macrocephala were found using multi-PCR reaction.The multi-PCR reaction system could be applied to identify A. macrocephala seed.