A 'bottom-counting' video system for measuring cocaine-induced behaviors in Drosophila

Cocaine exposure elicits a set of stereotypic behaviors in Drosophila that are strikingly similar to the cocaine-induced behaviors observed in vertebrates. This provides a valuable model for the study of cocaine abuse and has led to the discovery of a connection between the cocaine response pathway and the circadian system. This article describes a simplified assessment of cocaine-induced behavior combined with an image acquisition system, which allows the assay to be semiautomated. With this new system, cocaine response can be evaluated in a fraction of the time required by the previous assay, and subjectivity in scoring is reduced dramatically.