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Antigen-stimulated IL-4, IL-13 and IFN-gamma production by human T cells at a single-cell level
- Source :
- European journal of immunology. 28(10)
- Publication Year :
- 1998
-
Abstract
- To understand the intricate balance and the coordinate expression of the Th1 and Th2 cytokines following a natural mode of T cell triggering, antigen-stimulated IL-4, IL-13 and IFN-gamma production was studied in primary peripheral blood mononuclear cell cultures at a single-cell level. Cells from filariasis patients who respond to parasite antigen by producing not only IFN-gamma but also IL-4 and IL-13 were stimulated with Brugia malayi adult worm antigen and analyzed for co-expression of cytokines by intracellular staining. IL-4 and IL-13 were frequently co-expressed (54% of IL-4+ cells stained for IL-13 and 29% of IL-13+ cells expressed IL-4 at all time points), whereas IFN-gamma expression was totally segregated from both IL-4 and IL-13. These data indicate that in human peripheral T cells the co-expression of the dominant Th1 and Th2 cytokines within a single cell is a rare event and that IL-13 is clearly more frequently associated with a Th2 than a Th1 type response in primary T cell cultures.
Details
- ISSN :
- 00142980
- Volume :
- 28
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- European journal of immunology
- Accession number :
- edsair.pmid..........3d1fd6e38a7a75843ac4f546b4181470