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B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells
- Source :
- Methods in molecular biology (Clifton, N.J.). 1312
- Publication Year :
- 2015
-
Abstract
- The B-cell ELISPOT assay is a sensitive tool that can be utilized to measure total immunoglobulin (Ig) and antigen-specific antibody-secreting cells. Typically, membrane-bound antigen enables binding of antibody secreted by B-cells. Bound antibody is then detected by using an anti-Ig antibody and a colorimetric substrate, resulting in colored spots on the membrane that can be easily enumerated. Here we have described a method to quantitate antigen-specific antibody-secreting cells from the spleen or bone marrow of a vaccinated mouse.
Details
- ISSN :
- 19406029
- Volume :
- 1312
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Accession number :
- edsair.pmid..........3a91f74ef851b1fc186a880b7c7f2212