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[Sequencing and analysis of the full coding sequence of Batai virus isolated in China]
- Source :
- Bing du xue bao = Chinese journal of virology. 25(2)
- Publication Year :
- 2009
-
Abstract
- By RT-PCR and TAIL-PCR, the full coding region of Batai virus isolated in China (YN92-4 strain)was sequenced for the first time. According to the results, the genome of the virus contained three segments S, M and L of 947, 4,371 and 6,860 nucleotides, respectively. The S segment coded a nucleoprotein of 234 amino acids and a nonstructural protein of 102 amino acids, the M and L segments coded a precursor protein of 1 ,435 amino acids and RNA polymerase of 2,239 amino acids, respectively. Compared with the full coding sequence of Batai viruses isolated out of China, the S and M segments of YN92-4 and ON-7/B/01 showed the highest homology in nucleotide and amino acid sequenes with similarity of 97.7% (100%) and 95.7% (98%), respectively. Since there was no full coding sequence information on the L segment in GenBank for the reference, the L segment of YN92-4 was compared with that of Bunyamwera virus and the homology of nucleotide and amino acid was 73.5%and 81.6%, respectively. Phylogenetic analysis showed YN92-4 strain was clustered into one group with the prototype of Batai virus (MM2222). The results suggested that the YN92-4 strain had no occurrance of genetic reassortment (like Ngari virus) and was close to the Batai virus (ON-7/B/01 strain) isolated from cattle serum in Japan.
- Subjects :
- China
Base Sequence
Clinical Laboratory Techniques
Reverse Transcriptase Polymerase Chain Reaction
Molecular Sequence Data
Genome, Viral
Sequence Analysis, DNA
Polymerase Chain Reaction
Open Reading Frames
Hemorrhagic Fever with Renal Syndrome
Animals
Bunyamwera virus
Cattle
Amino Acid Sequence
Cloning, Molecular
Sequence Alignment
Reassortant Viruses
Subjects
Details
- ISSN :
- 10008721
- Volume :
- 25
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Bing du xue bao = Chinese journal of virology
- Accession number :
- edsair.pmid..........3746293c2c75c565d14267f4dbef977d